Intra Cytoplasmic Sperm Injection
ICSI is a type of assisted microsurgical fertilization that involves the injection of a single sperm directly into an egg. Eggs for ICSI are obtained in exactly the same way as those for IVF. Following the egg retrieval, the cells surrounding each egg are carefully removed. The eggs are then examined under a microscope and those that are judged as mature are suitable for injection. The sperm are washed and prepared. The egg and sperm are then placed on a special microscope that has micromanipulator attached to it. One micromanipulator holds the egg in place, while the other is used to inject the sperm into the egg. The remainder of the procedure is similar to standard IVF with regard to the incubation of the eggs and transfer of the resulting embryos.
Video on ICSI Procedure
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1.Very low numbers of motile sperm with normal appearance.
2.Problems with sperm binding and penetration.
3.Antisperm antibodies of sufficient quality to prevent fertilization.
4.Prior fertilization failure with standard IVF cycles and fertilization methods.
5.Absence of sperm in the seminal fluid